Comparison of rRNA-based reverse transcription PCR and rDNA-based PCR for the detection of streptococci in root canal infections

• Main Authors: Laís Cunha PRADO (Author), Giulio GAVINI (Author), Amanda da Costa SILVEIRA (Author), Vitor Cesar NAKAMURA (Author), Marcia Pinto Alves MAYER (Author), Ericka Tavares PINHEIRO (Author)
• Format: Book
• Published: University of São Paulo.
• Subjects: article
• Online Access: Connect to this object online.

Abstract Objective The rDNA-based method is unable to distinguish between alive and dead cells. Alternatively, bacterial viability can be assessed by molecular methods based on ribosomal RNA (rRNA). Therefore, this study aimed to detect viable streptococci in root canal samples using rRNA-based reve...