Gene Therapy for Neuropathic Pain through siRNA-IRF5 Gene Delivery with Homing Peptides to Microglia

• Main Authors: Tomoya Terashima (Author), Nobuhiro Ogawa (Author), Yuki Nakae (Author), Toshiyuki Sato (Author), Miwako Katagi (Author), Junko Okano (Author), Hiroshi Maegawa (Author), Hideto Kojima (Author)
• Format: Book
• Published: Elsevier, 2018-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Astrocyte- and microglia-targeting peptides were identified and isolated using phage display technology. A series of procedures, including three cycles of both in vivo and in vitro biopanning, was performed separately in astrocytes and in M1 or M2 microglia, yielding 50-58 phage plaques in each cell...