CCR5 Gene Editing of Resting CD4+ T Cells by Transient ZFN Expression From HIV Envelope Pseudotyped Nonintegrating Lentivirus Confers HIV-1 Resistance in Humanized Mice

• Main Authors: Guohua Yi (Author), Jang Gi Choi (Author), Preeti Bharaj (Author), Sojan Abraham (Author), Ying Dang (Author), Tal Kafri (Author), Ogechika Alozie (Author), Manjunath N Manjunath (Author), Premlata Shankar (Author)
• Format: Book
• Published: Elsevier, 2014-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

CCR5 disruption by zinc finger nucleases (ZFNs) is a promising method for HIV-1 gene therapy. However, successful clinical translation of this strategy necessitates the development of a safe and effective method for delivery into relevant cells. We used non-integrating lentivirus (NILV) for transien...