Silk/Fibroin Microcarriers for Mesenchymal Stem Cell Delivery: Optimization of Cell Seeding by the Design of Experiment

• Main Authors: Carlotta Perucca Orfei (Author), Giuseppe Talò (Author), Marco Viganò (Author), Sara Perteghella (Author), Gaia Lugano (Author), Francesca Fabro Fontana (Author), Enrico Ragni (Author), Alessandra Colombini (Author), Paola De Luca (Author), Matteo Moretti (Author), Maria Luisa Torre (Author), Laura de Girolamo (Author)
• Format: Book
• Published: MDPI AG, 2018-10-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

In this methodological paper, lyophilized fibroin-coated alginate microcarriers (LFAMs) proposed as mesenchymal stem cells (MSCs) delivery systems and optimal MSCs seeding conditions for cell adhesion rate and cell arrangement, was defined by a Design of Experiment (DoE) approach. Cells were co-incu...