Double-stranded RNA reduction by chaotropic agents during in vitro transcription of messenger RNA

• Main Authors: Xijun Piao (Author), Vibha Yadav (Author), Eddie Wang (Author), Wayne Chang (Author), Lanna Tau (Author), Benjamin E. Lindenmuth (Author), Sharon X. Wang (Author)
• Format: Book
• Published: Elsevier, 2022-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

In-vitro-transcribed messenger RNA (mRNA) has recently shown increasing significance in the development of vaccines and therapeutics. Immunogenic double-stranded RNA (dsRNA) is an undesired byproduct formed during in vitro transcription (IVT), and it is challenging to reduce dsRNA byproduct from mRN...