Double-stranded RNA reduction by chaotropic agents during in vitro transcription of messenger RNA

• Главные авторы: Xijun Piao (Автор), Vibha Yadav (Автор), Eddie Wang (Автор), Wayne Chang (Автор), Lanna Tau (Автор), Benjamin E. Lindenmuth (Автор), Sharon X. Wang (Автор)
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• Опубликовано: Elsevier, 2022-09-01T00:00:00Z.
• Предметы: article
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In-vitro-transcribed messenger RNA (mRNA) has recently shown increasing significance in the development of vaccines and therapeutics. Immunogenic double-stranded RNA (dsRNA) is an undesired byproduct formed during in vitro transcription (IVT), and it is challenging to reduce dsRNA byproduct from mRN...