Development and evaluation of a sensitive PCR-ELISA system for detection of schistosoma infection in feces.

• Main Authors: Luciana Inácia Gomes (Author), Letícia Helena Dos Santos Marques (Author), Martin Johannes Enk (Author), Maria Cláudia de Oliveira (Author), Paulo Marcos Zech Coelho (Author), Ana Rabello (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2010-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

BACKGROUND: A PCR-enzyme-linked immunosorbent assay (PCR-ELISA) was developed to overcome the need for sensitive techniques for the efficient diagnosis of Schistosoma infection in endemic settings with low parasitic burden. METHODOLOGY/PRINCIPAL FINDINGS: This system amplifies a 121-base pair tandem...