Comparative Study of Culture Conditions for Maintaining CYP3A4 and ATP-Binding Cassette Transporters Activity in Primary Cultured Human Hepatocytes

• Main Authors: Yuko Takeba (Author), Naoki Matsumoto (Author), Sachiko Takenoshita-Nakaya (Author), Yoshie Harimoto (Author), Toshio Kumai (Author), Yuichi Kinoshita (Author), Hiroshi Nakano (Author), Takehito Ohtsubo (Author), Shinichi Kobayashi (Author)
• Format: Book
• Published: Elsevier, 2011-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The aim of this study was to determine suitable culture conditions for maintaining the activity of cytochrome p450 (CYP) 3A4 and drug transporters in primary cultured human hepatocytes. Human hepatocytes were isolated using the two-step collagenase perfusion technique and were cultured with four dif...