A Simple Cloning-free Method to Efficiently Induce Gene Expression Using CRISPR/Cas9
Gain-of-function studies often require the tedious cloning of transgene cDNA into vectors for overexpression beyond the physiological expression levels. The rapid development of CRISPR/Cas technology presents promising opportunities to address these issues. Here, we report a simple, cloning-free met...
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Main Authors: | , , , , , , , , |
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Format: | Bog |
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Elsevier,
2019-03-01T00:00:00Z.
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A1234.567 |
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