A Simple Cloning-free Method to Efficiently Induce Gene Expression Using CRISPR/Cas9

• Main Authors: Lyujie Fang (Author), Sandy S.C. Hung (Author), Jennifer Yek (Author), Layal El Wazan (Author), Tu Nguyen (Author), Shahnaz Khan (Author), Shiang Y. Lim (Author), Alex W. Hewitt (Author), Raymond C.B. Wong (Author)
• Format: Book
• Published: Elsevier, 2019-03-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Gain-of-function studies often require the tedious cloning of transgene cDNA into vectors for overexpression beyond the physiological expression levels. The rapid development of CRISPR/Cas technology presents promising opportunities to address these issues. Here, we report a simple, cloning-free met...