A Simple Cloning-free Method to Efficiently Induce Gene Expression Using CRISPR/Cas9

• Главные авторы: Lyujie Fang (Автор), Sandy S.C. Hung (Автор), Jennifer Yek (Автор), Layal El Wazan (Автор), Tu Nguyen (Автор), Shahnaz Khan (Автор), Shiang Y. Lim (Автор), Alex W. Hewitt (Автор), Raymond C.B. Wong (Автор)
• Формат: ‌
• Опубликовано: Elsevier, 2019-03-01T00:00:00Z.
• Предметы: article
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Gain-of-function studies often require the tedious cloning of transgene cDNA into vectors for overexpression beyond the physiological expression levels. The rapid development of CRISPR/Cas technology presents promising opportunities to address these issues. Here, we report a simple, cloning-free met...