Bioluminescent imaging of Trypanosoma brucei shows preferential testis dissemination which may hamper drug efficacy in sleeping sickness.

• Main Authors: Filip Claes (Author), Suman K Vodnala (Author), Nick van Reet (Author), Nathalie Boucher (Author), Hilda Lunden-Miguel (Author), Theo Baltz (Author), Bruno Maria Goddeeris (Author), Philippe Büscher (Author), Martin E Rottenberg (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2009-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Monitoring Trypanosoma spread using real-time imaging in vivo provides a fast method to evaluate parasite distribution especially in immunoprivileged locations. Here, we generated monomorphic and pleomorphic recombinant Trypanosoma brucei expressing the Renilla luciferase. In vitro luciferase activi...