Identification and characterization of an unexpected isomerization motif in CDRH2 that affects antibody activity

• Main Authors: Meiqi Yi (Author), Jian Sun (Author), Hanzi Sun (Author), Yifei Wang (Author), Shan Hou (Author), Beibei Jiang (Author), Yuanyuan Xie (Author), Ruyue Ji (Author), Liu Xue (Author), Xiao Ding (Author), Xiaomin Song (Author), April Xu (Author), Chichi Huang (Author), Quan Quan (Author), Jing Song (Author)
• Format: Book
• Published: Taylor & Francis Group, 2023-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

ABSTRACTAspartic acid (Asp) isomerization is a spontaneous non-enzymatic post-translation modification causing a change in the structure of the protein backbone, which is commonly observed in therapeutic antibodies during manufacturing and storage. The Asps in Asp-Gly (DG), Asp-Ser (DS), and Asp-Thr...