PCR-RFLP analyses of Leishmania species causing cutaneous and mucocutaneous leishmaniasis revealed distribution of genetically complex strains with hybrid and mito-nuclear discordance in Ecuador.

• Main Authors: Hirotomo Kato (Author), Eduardo A Gomez (Author), Chisato Seki (Author), Hayato Furumoto (Author), Luiggi Martini-Robles (Author), Jenny Muzzio (Author), Manuel Calvopiña (Author), Lenin Velez (Author), Makoto Kubo (Author), Ahmed Tabbabi (Author), Daisuke S Yamamoto (Author), Yoshihisa Hashiguchi (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2019-05-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

PCR-Restriction Fragment Length Polymorphism (RFLP) analyses targeting multiple nuclear genes were established for the simple and practical identification of Leishmania species without using expensive equipment. This method was applied to 92 clinical samples collected at 33 sites in 14 provinces of...