Central composite design expert-supported development and validation of HPTLC method: Relevance in quantitative evaluation of protopine in Fumaria indica

• Main Authors: Ajaz Ahmad (Author), Mohd Amir (Author), Abdulrahman A. Alshadidi (Author), Muhammad Delwar Hussain (Author), Anzarul Haq (Author), Mohsin Kazi (Author)
• Format: Book
• Published: Elsevier, 2020-04-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The current study was executed for method development, validation and to estimate the concentration of protopine in methanolic extract of Fumaria indica by high-performance thin-layer chromatography (HPTLC). Isolation of bioactive compounds was carried out using the mobile phase, toluene:ethyl aceta...