Primary vaccination with low dose live dengue 1 virus generates a proinflammatory, multifunctional T cell response in humans.

• Հիմնական հեղինակներ: Janet C Lindow (Հեղինակ), Nathan Borochoff-Porte (Հեղինակ), Anna P Durbin (Հեղինակ), Stephen S Whitehead (Հեղինակ), Kelly A Fimlaid (Հեղինակ), Janice Y Bunn (Հեղինակ), Beth D Kirkpatrick (Հեղինակ)
• Ձևաչափ: Գիրք
• Հրապարակվել է: Public Library of Science (PLoS), 2012-01-01T00:00:00Z.
• Խորագրեր: article
• Առցանց հասանելիություն: Connect to this object online.

Ամփոփում

The four dengue virus serotypes (DENV-1-DENV-4) have a large impact on global health, causing 50-100 million cases of dengue fever annually. Herein, we describe the first kinetic T cell response to a low-dose DENV-1 vaccination study (10 PFU) in humans. Using flow cytometry, we found that proinflammatory cytokines, IFNγ, TNFα, and IL-2, were generated by DENV-1-specific CD4(+) cells 21 days post-DENV-1 exposure, and their production continued through the latest time-point, day 42 (p<0.0001 for all cytokines). No statistically significant changes were observed at any time-points for IL-10 (p = 0.19), a regulatory cytokine, indicating that the response to DENV-1 was primarily proinflammatory in nature. We also observed little T cell cross-reactivity to the other 3 DENV serotypes. The percentage of multifunctional T cells (T cells making ≥ 2 cytokines simultaneously) increased with time post-DENV-1 exposure (p<0.0001). The presence of multifunctional T cells together with neutralizing antibody data suggest that the immune response generated to the vaccine may be protective. This work provides an initial framework for defining primary T cell responses to each DENV serotype and will enhance the evaluation of a tetravalent DENV vaccine.

Նյութի նկարագրություն

1935-2727
1935-2735
10.1371/journal.pntd.0001742