Combinatorial mutation on the β-glycosidase specific to 7-β-xylosyltaxanes and increasing the mutated enzyme production by engineering the recombinant yeast

• Main Authors: Jing-Jing Chen (Author), Xiao Liang (Author), Fen Wang (Author), Yan-Hua Wen (Author), Tian-Jiao Chen (Author), Wan-Cang Liu (Author), Ting Gong (Author), Jin-Ling Yang (Author), Ping Zhu (Author)
• Format: Bog
• Udgivet: Elsevier, 2019-05-01T00:00:00Z.
• Fag: article
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Taxol is a "blockbuster" antitumor drug produced by Taxus species with extremely low amount, while its analogue 7-β-xylosyl-10-deacetyltaxol is generally much higher in the plants. Both the fungal enzymes LXYL-P1−1 and LXYL-P1−2 can convert 7-β-xylosyl-10-deacetyltaxol into 10-deacetyltaxo...