Determination of ergocalciferol in human plasma after Diels-Alder derivatization by LCâMS/MS and its application to a bioequivalence study

• Main Authors: Pritesh Contractor (Author), Abhishek Gandhi (Author), Gajendra Solanki (Author), Priyanka A. Shah (Author), Pranav S. Shrivastav (Author)
• Format: Book
• Published: Elsevier, 2017-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

An accurate, sensitive and selective method is developed for determination of ergocalciferol (vitamin D2) in human plasma using LCâMS/MS. After liquid-liquid extraction with n-hexane, ergocalciferol was derivatized by reacting with 4-phenyl-1,2,4-triazoline-3,5-dione (PTAD), a strong dienophile base...