Determination of ergocalciferol in human plasma after Diels-Alder derivatization by LCâMS/MS and its application to a bioequivalence study
An accurate, sensitive and selective method is developed for determination of ergocalciferol (vitamin D2) in human plasma using LCâMS/MS. After liquid-liquid extraction with n-hexane, ergocalciferol was derivatized by reacting with 4-phenyl-1,2,4-triazoline-3,5-dione (PTAD), a strong dienophile base...
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| Main Authors: | , , , , |
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| Format: | Book |
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Elsevier,
2017-12-01T00:00:00Z.
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