Real-time isothermal nucleic acid amplification detection in resource-limited settings: a description of an open-source miniature fluorimeter

• Main Authors: Jackson Coole, BS (Author), Alex Kortum, MSc (Author), Yubo Tang, PhD (Author), Imran Vohra, BS (Author), Mary Natoli, PhD (Author), Kathryn Kundrod, PhD (Author), Rebecca Richards-Kortum, PhD (Author)
• Format: Book
• Published: Elsevier, 2021-03-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Background: The detection and quantification of nucleic acids traditionally relies on PCR, which requires the use of expensive thermocyclers with integrated fluorescence detection of amplicons. While isothermal nucleic acid amplification technologies eliminate the need for thermocycling, fluorescenc...