A more efficient CRISPR-Cas12a variant derived from Lachnospiraceae bacterium MA2020

• Main Authors: Mai H. Tran (Author), Hajeung Park (Author), Christopher L. Nobles (Author), Pabalu Karunadharma (Author), Li Pan (Author), Guocai Zhong (Author), Haimin Wang (Author), Wenhui He (Author), Tianling Ou (Author), Gogce Crynen (Author), Kelly Sheptack (Author), Ian Stiskin (Author), Huihui Mou (Author), Michael Farzan (Author)
• Format: Book
• Published: Elsevier, 2021-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

CRISPR effector proteins introduce double-stranded breaks into the mammalian genome, facilitating gene editing by non-homologous end-joining or homology-directed repair. Unlike the more commonly studied Cas9, the CRISPR effector protein Cas12a/Cpf1 recognizes a T-rich protospacer adjacent motif (PAM...