Enhanced Prokaryotic Expression of Dengue Virus Envelope Protein

• Main Authors: Advaita Ganguly (Author), Ravindra B. Malabadi (Author), Dipankar Das (Author), Mavanur R. Suresh (Author), Hoon H Sunwoo (Author)
• Format: Book
• Published: Frontiers Media S.A., 2013-10-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Purpose. To highlight the expression and purification of the recombinant dengue virus type-1 antigen exploiting the codon optimized full length envelope for increased yield in E. coli. Methods. A 6x His tag was inserted at the C terminus to facilitate purification. The purified protein was recognize...