Comparison of methylation estimates obtained via MinION nanopore sequencing and sanger bisulfite sequencing in the TRPA1 promoter region

• Main Authors: Sara Gombert (Author), Kirsten Jahn (Author), Hansi Pathak (Author), Alexandra Burkert (Author), Gunnar Schmidt (Author), Lutz Wiehlmann (Author), Colin Davenport (Author), Björn Brändl (Author), Franz-Josef Müller (Author), Andreas Leffler (Author), Maximilian Deest (Author), Helge Frieling (Author)
• Format: Book
• Published: BMC, 2023-10-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Abstract Background Bisulfite sequencing has long been considered the gold standard for measuring DNA methylation at single CpG resolution. However, in recent years several new approaches like nanopore sequencing have been developed due to hints for a partial error-proneness of bisulfite sequencing....