FIB/SEM technology and high-throughput 3D reconstruction of dendritic spines and synapses in GFP-labeled adult-generated neurons

• मुख्य लेखकों: Carles eBosch (लेखक), Albert eMartínez (लेखक), Núria eMasachs (लेखक), Cátia M Teixeira (लेखक), Isabel eFernaud (लेखक), Fausto eUlloa (लेखक), Esther ePérez (लेखक), Carlos eLois (लेखक), Joan X. Comella (लेखक), Javier eDeFelipe (लेखक), Angel eMerchan-Perez (लेखक), Eduardo eSoriano (लेखक)
• स्वरूप: पुस्तक
• प्रकाशित: Frontiers Media S.A., 2015-05-01T00:00:00Z.
• विषय: article
• ऑनलाइन पहुंच: Connect to this object online.

The fine analysis of synaptic contacts is usually performed using transmission electron microscopy (TEM) and its combination with neuronal labeling techniques. However, the complex 3D architecture of neuronal samples calls for their reconstruction from serial sections. Here we show that focused ion...