FIB/SEM technology and high-throughput 3D reconstruction of dendritic spines and synapses in GFP-labeled adult-generated neurons

• Main Authors: Carles eBosch (Author), Albert eMartínez (Author), Núria eMasachs (Author), Cátia M Teixeira (Author), Isabel eFernaud (Author), Fausto eUlloa (Author), Esther ePérez (Author), Carlos eLois (Author), Joan X. Comella (Author), Javier eDeFelipe (Author), Angel eMerchan-Perez (Author), Eduardo eSoriano (Author)
• Format: Book
• Published: Frontiers Media S.A., 2015-05-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The fine analysis of synaptic contacts is usually performed using transmission electron microscopy (TEM) and its combination with neuronal labeling techniques. However, the complex 3D architecture of neuronal samples calls for their reconstruction from serial sections. Here we show that focused ion...