A Microneedle-Microplate Platform to Detect Biomarkers in the Skin
This research aimed to integrate an established signal quantification technique with immunocapture microneedle arrays (MNAs) to improve biomarker-based disease diagnostics in the skin. In this miniaturized sandwich enzyme-linked immunosorbent assay (ELISA) platform, the capture antibody was immobili...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Book |
| Published: |
University of Huddersfield Press,
2022-11-01T00:00:00Z.
|
| Subjects: | |
| Online Access: | Connect to this object online. |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | This research aimed to integrate an established signal quantification technique with immunocapture microneedle arrays (MNAs) to improve biomarker-based disease diagnostics in the skin. In this miniaturized sandwich enzyme-linked immunosorbent assay (ELISA) platform, the capture antibody was immobilised onto the surface of microneedles which, when inserted superficially into porcine skin, captured endogenous porcine immunoglobulin G (IgG) intradermally. Signal development was achieved by incubating the microneedles into-phenylenediamine (OPD) solution in a 384-well microplate and measuring the absorbance in a microplate spectrophotometer at 450 nm. This technique allows for rapid biomarker detection with high-throughput processing. Immunocapture microneedle devices such as these can be easily adapted for targeting different biomarkers or multiplexed leaving plenty of scope for future work and assay optimisation. |
|---|---|
| Item Description: | 10.5920/bjpharm.1164 2058-8356 |