The impact of proline isomerization on antigen binding and the analytical profile of a trispecific anti-HIV antibody

• Main Authors: Alessandro Masiero (Author), Lechat Nelly (Author), Gentric Marianne (Author), Sourrouille Christophe (Author), Laville Florian (Author), Crépin Ronan (Author), Borel Claire (Author), Ziegler Cornelia (Author), Bisch Grégoire (Author), Leclerc Eric (Author), Laurent Ludovic (Author), Brault Dominique (Author), Alexandre Sylvie (Author), Gagnaire Marie (Author), Duffieux Francis (Author), Soubrier Fabienne (Author), Capdevila Cécile (Author), Arnould Isabelle (Author), Dumas Jacques (Author), Dabin Jérôme (Author), Genet Bruno (Author), Radošević Katarina (Author), Menet Jean-Michel (Author), Prades Catherine (Author)
• Format: Book
• Published: Taylor & Francis Group, 2020-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Proline cis-trans conformational isomerization is a mechanism that affects different types of protein functions and behaviors. Using analytical characterization, structural analysis, and molecular dynamics simulations, we studied the causes of an aberrant two-peak size-exclusion chromatography profi...