Development of a LC-ESI-MRM method for the absolute quantification of orexin A in the CSF of individual mice

• Main Authors: Katherine Hopkins (Author), Soumya Mukherjee (Author), Dalia Ponce (Author), Jonathan Mangum (Author), Laura H. Jacobson (Author), Daniel Hoyer (Author)
• Format: Book
• Published: Elsevier, 2021-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Sleep-wakefulness is disrupted in most neurological and psychiatric disorders. Although clinical data implicate orexin (hypocretin), a crucial sleep/wake regulatory neuropeptide, in such disorders, limited sample volumes effectively prevent quantification of cerebrospinal fluid (CSF) levels of orexi...