Development and evaluation of antibody-capture immunoassays for detection of Lassa virus nucleoprotein-specific immunoglobulin M and G.

• Main Authors: Martin Gabriel (Author), Donatus I Adomeh (Author), Jacqueline Ehimuan (Author), Jennifer Oyakhilome (Author), Emmanuel O Omomoh (Author), Yemisi Ighodalo (Author), Thomas Olokor (Author), Kofi Bonney (Author), Meike Pahlmann (Author), Petra Emmerich (Author), Michaela Lelke (Author), Linda Brunotte (Author), Stephan Ölschläger (Author), Corinna Thomé-Bolduan (Author), Beate Becker-Ziaja (Author), Carola Busch (Author), Ikponmwosa Odia (Author), Ephraim Ogbaini-Emovon (Author), Peter O Okokhere (Author), Sylvanus A Okogbenin (Author), George O Akpede (Author), Herbert Schmitz (Author), Danny A Asogun (Author), Stephan Günther (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2018-03-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The classical method for detection of Lassa virus-specific antibodies is the immunofluorescence assay (IFA) using virus-infected cells as antigen. However, IFA requires laboratories of biosafety level 4 for assay production and an experienced investigator to interpret the fluorescence signals. There...