Influence of amplicon size on the polymerase chain reaction of Parvovirus B19 genome in formalin-fixed specimens Influência do tamanho do amplicon na reação em cadeia da polimerase na detecção do genoma do PB19 em amostras fixadas em formalina

• Glavni autori: Paulo Roberto Veiga Quemelo (Autor), Benedito Antônio Lopes da Fonseca (Autor), Danielle Malta Lima (Autor), Luiz Cesar Peres (Autor)
• Format: Knjiga
• Izdano: Sociedade Brasileira de Patologia Clínica, 2009-04-01T00:00:00Z.
• Teme: article
• Online pristup: Connect to this object online.

The polymerase chain reaction (PCR) has provided diagnosis of archival material, but some fixation methods such as formalin damage DNA and, subsequently, affect PCR analysis, particularly paraffin-embedded tissues. PCR is known due to its high specificity and sensitivity, although some difficulties...