Development of an active-site titrant for SARS-CoV-2 main protease as an indispensable tool for evaluating enzyme kinetics

• Main Authors: Rabea Voget (Author), Julian Breidenbach (Author), Tobias Claff (Author), Alexandra Hingst (Author), Katharina Sylvester (Author), Christian Steinebach (Author), Lan Phuong Vu (Author), Renato H. Weiße (Author), Ulrike Bartz (Author), Norbert Sträter (Author), Christa E. Müller (Author), Michael Gütschow (Author)
• Format: Book
• Published: Elsevier, 2024-05-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

A titrant for the SARS-CoV-2 main protease (Mpro) was developed that enables, for the first time, the exact determination of the concentration of the enzymatically active Mpro by active-site titration. The covalent binding mode of the tetrapeptidic titrant was elucidated by the determination of the...