Radiolabeled 15-mer peptide internalization is mediated by megalin (LRP2 receptor) in a CRISPR/Cas9-based LRP2 knockout human kidney cell model

• Main Authors: Anna Durinova (Author), Lucie Smutna (Author), Pavel Barta (Author), Rajamanikkam Kamaraj (Author), Tomas Smutny (Author), Bernhard Schmierer (Author), Petr Pavek (Author), Frantisek Trejtnar (Author)
• Format: Book
• Published: SpringerOpen, 2024-04-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Abstract Background Megalin (LRP2 receptor) mediates the endocytosis of radiolabeled peptides into proximal tubular kidney cells, which may cause nephrotoxicity due to the accumulation of a radioactive tracer. The study aimed to develop a cellular model of human kidney HK2 cells with LRP2 knockout (...