In-cell bioluminescence resonance energy transfer (BRET)-based assay uncovers ceritinib and CA-074 as SARS-CoV-2 papain-like protease inhibitors

• Main Authors: Mei Li (Author), Zhu-Chun Bei (Author), Yongtian Yuan (Author), Baogang Wang (Author), Dongna Zhang (Author), Likun Xu (Author), Liangliang Zhao (Author), Qin Xu (Author), Yabin Song (Author)
• Format: Book
• Published: Taylor & Francis Group, 2024-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Papain-like protease (PLpro) is an attractive anti-coronavirus target. The development of PLpro inhibitors, however, is hampered by the limitations of the existing PLpro assay and the scarcity of validated active compounds. We developed a novel in-cell PLpro assay based on BRET and used it to evalua...