Self-replicating Replicon-RNA Delivery to Dendritic Cells by Chitosan-nanoparticles for Translation In Vitro and In Vivo

• Main Authors: Kenneth C McCullough (Author), Isabelle Bassi (Author), Panagiota Milona (Author), Rolf Suter (Author), Lisa Thomann-Harwood (Author), Pavlos Englezou (Author), Thomas Démoulins (Author), Nicolas Ruggli (Author)
• Format: Book
• Published: Elsevier, 2014-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Self-amplifying replicon RNA (RepRNA) possesses high potential for increasing antigen load within dendritic cells (DCs). The major aim of the present work was to define how RepRNA delivered by biodegradable, chitosan-based nanoparticulate delivery vehicles (nanogel-alginate (NGA)) interacts with DCs...