CRISPA: A Non-viral, Transient Cas9 Delivery System Based on Reengineered Anthrax Toxin

• Main Authors: Maximilian Hirschenberger (Author), Nicole Stadler (Author), Maximilian Fellermann (Author), Konstantin M. J. Sparrer (Author), Frank Kirchhoff (Author), Holger Barth (Author), Panagiotis Papatheodorou (Author)
• Format: Book
• Published: Frontiers Media S.A., 2021-10-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Translating the CRISPR/Cas9 genome editing technology into clinics is still hampered by rather unspecific, unsafe and/or inconvenient approaches for the delivery of its main components - the Cas9 endonuclease and a guide RNA - into cells. Here, we describe the development of a novel transient and no...