Improved Lentiviral Gene Delivery to Mouse Liver by Hydrodynamic Vector Injection through Tail Vein

• Main Authors: Trine Dalsgaard (Author), Claudia R. Cecchi (Author), Anne Louise Askou (Author), Rasmus O. Bak (Author), Pernille O. Andersen (Author), David Hougaard (Author), Thomas G. Jensen (Author), Frederik Dagnæs-Hansen (Author), Jacob Giehm Mikkelsen (Author), Thomas J. Corydon (Author), Lars Aagaard (Author)
• Format: Book
• Published: Elsevier, 2018-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Delivery of genes to mouse liver is routinely accomplished by tail-vein injections of viral vectors or naked plasmid DNA. While viral vectors are typically injected in a low-pressure and -volume fashion, uptake of naked plasmid DNA to hepatocytes is facilitated by high pressure and volumes, also kno...