Metabolic profiling of single cells by exploiting NADH and FAD fluorescence via flow cytometry

• Main Authors: Ariful Haque Abir (Author), Leonie Weckwerth (Author), Artur Wilhelm (Author), Jana Thomas (Author), Clara M. Reichardt (Author), Luis Munoz (Author), Simon Völkl (Author), Uwe Appelt (Author), Markus Mroz (Author), Raluca Niesner (Author), Anja Hauser (Author), Rebecca Sophie Fischer (Author), Katharina Pracht (Author), Hans-Martin Jäck (Author), Georg Schett (Author), Gerhard Krönke (Author), Dirk Mielenz (Author)
• Format: Book
• Published: Elsevier, 2024-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Objective: The metabolism of different cells within the same microenvironment can differ and dictate physiological or pathological adaptions. Current single-cell analysis methods of metabolism are not label-free. Methods: The study introduces a label-free, live-cell analysis method assessing endogen...