ELISA- and Activity Assay-Based Quantification of BMP-2 Released <i>In Vitro</i> Can Be Biased by Solubility in "Physiological" Buffers and an Interfering Effect of Chitosan

• Main Authors: Julius Sundermann (Author), Steffen Sydow (Author), Laura Burmeister (Author), Andrea Hoffmann (Author), Henning Menzel (Author), Heike Bunjes (Author)
• Format: Book
• Published: MDPI AG, 2021-04-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Chitosan nanogel-coated polycaprolactone (PCL) fiber mat-based implant prototypes with tailored release of bone morphogenic protein 2 (BMP-2) are a promising approach to achieve implant-mediated bone regeneration. In order to ensure reliable <i>in vitro</i> release results, the robustnes...