The construction of in vitro nasal cavity-mimic M-cell model, design of M cell-targeting nanoparticles and evaluation of mucosal vaccination by nasal administration

• Main Authors: Xiaotong Yang (Author), Xianchun Chen (Author), Ting Lei (Author), Lin Qin (Author), Yang Zhou (Author), Chuan Hu (Author), Qingfeng Liu (Author), Huile Gao (Author)
• Format: Book
• Published: Elsevier, 2020-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

In order to better evaluate the transport effect of nanoparticles through the nasal mucosa, an in vitro nasal cavity-mimic model was designed based on M cells. The differentiation of M cells was induced by co-culture of Calu-3 and Raji cells in invert model. The ZO-1 protein staining and the transpo...