Comparison of microbiological assay and HPLC-UV for determination of fluconazole in capsules

• Main Authors: Kelly Marques Queiroz (Author), Maria-Luiza Martins Silva (Author), Nathália Duque Prado (Author), Paulo Marcelo Andrade Lima (Author), Rosiane Dias Lopes Diniz (Author), Isabela Costa César (Author), Gerson Antônio Pianetti (Author), Daniel Assis Santos (Author)
• Format: Book
• Published: Universidade de São Paulo, 2009-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The development of a specific agar diffusion bioassay for the quantitative determination of fluconazole formulated in capsules was carried out using a strain of Candida albicans ATCC 18804 as the test organism. A prospective validation of the method showed adequate linearity (r²=0.9995), precision (...