The enhancement of M13 phage titration by optimizing the origin of replication
Background and purpose: M13KO7, a modified M13 phage variant, carries the p15A replication origin and Tn903 kanamycin resistance gene. This study aimed to optimize M13KO7's replication by substituting the p15A origin with the higher-copy pMB1 origin (500-700 copy numbers). Experimental approach...
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| Formaat: | Boek |
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Wolters Kluwer Medknow Publications,
2024-07-01T00:00:00Z.
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| Plaatsingsnummer: |
A1234.567 |
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| Kopie 1 | Beschikbaar |