The enhancement of M13 phage titration by optimizing the origin of replication

Background and purpose: M13KO7, a modified M13 phage variant, carries the p15A replication origin and Tn903 kanamycin resistance gene. This study aimed to optimize M13KO7's replication by substituting the p15A origin with the higher-copy pMB1 origin (500-700 copy numbers). Experimental approach...

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Main Authors: Mohammad Hossein Darvishali (Author), Mahmood Fadaie (Author), Hossein Khanahmad (Author)
Format: Book
Published: Wolters Kluwer Medknow Publications, 2024-07-01T00:00:00Z.
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3rd Floor Main Library

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Call Number: A1234.567
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