Accurate real-time PCR strategy for monitoring bloodstream parasitic loads in chagas disease patients.

• Main Authors: Tomas Duffy (Author), Margarita Bisio (Author), Jaime Altcheh (Author), Juan Miguel Burgos (Author), Mirta Diez (Author), Mariano Jorge Levin (Author), Roberto Rene Favaloro (Author), Hector Freilij (Author), Alejandro Gabriel Schijman (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2009-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

<h4>Background</h4>This report describes a real-time PCR (Q-PCR) strategy to quantify Trypanosoma cruzi (T. cruzi) DNA in peripheral blood samples from Chagas disease patients targeted to conserved motifs within the repetitive satellite sequence.<h4>Methodology/principal findings&l...