Application of human induced pluripotent stem cell-derived cardiomyocytes sheets with microelectrode array system to estimate antiarrhythmic properties of multi-ion channel blockers
We examined electrophysiological indices of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) sheets in order to quantitatively estimate Na+, K+ and Ca2+ channel blocking actions of bepridil and amiodarone using microelectrode array system in comparison with that of E-4031. We a...
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Elsevier,
2018-08-01T00:00:00Z.
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| 001 | doaj_5f801f9b043947e7b986f49b18cd13e1 | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Hiroko Izumi-Nakaseko |e author |
| 700 | 1 | 0 | |a Mihoko Hagiwara-Nagasawa |e author |
| 700 | 1 | 0 | |a Atsuhiko T. Naito |e author |
| 700 | 1 | 0 | |a Ai Goto |e author |
| 700 | 1 | 0 | |a Koki Chiba |e author |
| 700 | 1 | 0 | |a Yuko Sekino |e author |
| 700 | 1 | 0 | |a Yasunari Kanda |e author |
| 700 | 1 | 0 | |a Atsushi Sugiyama |e author |
| 245 | 0 | 0 | |a Application of human induced pluripotent stem cell-derived cardiomyocytes sheets with microelectrode array system to estimate antiarrhythmic properties of multi-ion channel blockers |
| 260 | |b Elsevier, |c 2018-08-01T00:00:00Z. | ||
| 500 | |a 1347-8613 | ||
| 500 | |a 10.1016/j.jphs.2018.07.011 | ||
| 520 | |a We examined electrophysiological indices of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) sheets in order to quantitatively estimate Na+, K+ and Ca2+ channel blocking actions of bepridil and amiodarone using microelectrode array system in comparison with that of E-4031. We analyzed the field potential duration, effective refractory period, current threshold and conduction property using a programmed electrical stimulation protocol to obtain the post repolarization refractoriness and coefficient a of the relationship between the pacing cycle length and field potential duration. Electropharmacological profile of each drug was successfully characterized; namely, 1) the changes in the current threshold and conduction property provided basic information of Na+ channel blocking kinetics, 2) the relationship between pacing cycle length and field potential duration reflected drug-induced inhibition of human ether-à-go-go-related gene (hERG) K+ channel, 3) the post repolarization refractoriness indicated the relative contribution of these drugs to Na+ and K+ channel blockade, and 4) L-type Ca2+ channel blocking action was more obvious in the field potential waveform of the hiPSC-CMs sheets than that expected in the electrocardiogram in humans. Thus, this information may help to better utilize the hiPSC-CMs sheets for grasping the properties and net effects of drug-induced Na+, Ca2+ and K+ channel blockade. Keywords: Human induced pluripotent stem cell-derived cardiomyocytes, Post repolarization refractoriness, Multichannel blocker, Antiarrhythmic property | ||
| 546 | |a EN | ||
| 690 | |a Therapeutics. Pharmacology | ||
| 690 | |a RM1-950 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n Journal of Pharmacological Sciences, Vol 137, Iss 4, Pp 372-378 (2018) | |
| 787 | 0 | |n http://www.sciencedirect.com/science/article/pii/S1347861318301312 | |
| 787 | 0 | |n https://doaj.org/toc/1347-8613 | |
| 856 | 4 | 1 | |u https://doaj.org/article/5f801f9b043947e7b986f49b18cd13e1 |z Connect to this object online. |