Optimization of methods to assess mitochondrial DNA in archival paraffin-embedded tissues from mammary canine tumors Otimização dos métodos para avaliar o DNA mitocondrial obtido a partir de tumores mamários caninos incluídos em parafina

• Main Authors: Angélica C. Bertagnolli (Author), Valdemar Máximo (Author), Bárbara van Asch (Author), António Amorim (Author), Luis Cirnes (Author), Paula Soares (Author), Geovanni D. Cassali (Author)
• Format: Book
• Published: Sociedade Brasileira de Patologia Clínica, 2008-08-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

In this study we describe the alterations used to extract and amplify mitochondrial desoxyribonucleic acid (DNA) from formalin-fixed paraffin-embedded samples of canine mammary tumors. The epithelial and mesenchymal components (chondromyxoid and chondroid) of each tumor, as well as the normal mammar...