Chaperone-Assisted Soluble Expression of a Humanized Anti-EGFR ScFv Antibody in E. Coli

• Main Authors: Kamal Veisi (Author), Safar Farajnia (Author), Nosratollah Zarghami (Author), Hamid Reza Khoram Khorshid (Author), Nasser Samadi (Author), Shiva Ahdi Khosroshahi (Author), Hossein Zarei Jaliani (Author)
• Format: Book
• Published: Tabriz University of Medical Sciences, 2015-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Purpose: Formation of inclusion bodies is a considerable obstacle threatening the advantages of E. coli expression system to serve as the most common and easiest system in recombinant protein production. To solve this problem, several strategies have been proposed among which application of molecula...