Validation and Application of a Commercial Quantitative Real-Time Reverse Transcriptase-PCR Assay in Investigation of a Large Dengue Virus Outbreak in Southern Taiwan.

• Main Authors: Huey-Pin Tsai (Author), You-Yuan Tsai (Author), I-Ting Lin (Author), Pin-Hwa Kuo (Author), Kung-Chao Chang (Author), Jung-Chin Chen (Author), Wen-Chien Ko (Author), Jen-Ren Wang (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2016-10-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

BACKGROUND:Accurate, rapid, and early diagnosis of dengue virus (DENV) infections is essential for optimal clinical care. Here, we evaluated the efficacy of the quantitative real-time PCR (qRT-PCR)-LightMix dengue virus EC kit for DENV detection using samples from a dengue outbreak in Taiwan in 2015...