Utilization of proliferable extracellular amastigotes for transient gene expression, drug sensitivity assay, and CRISPR/Cas9-mediated gene knockout in Trypanosoma cruzi.

Trypanosoma cruzi has three distinct life cycle stages; epimastigote, trypomastigote, and amastigote. Amastigote is the replication stage in host mammalian cells, hence this stage of parasite has clinical significance in drug development research. Presence of extracellular amastigotes (EA) and their...

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Main Authors: Yuko Takagi (Author), Yukie Akutsu (Author), Motomichi Doi (Author), Koji Furukawa (Author)
Format: Book
Published: Public Library of Science (PLoS), 2019-01-01T00:00:00Z.
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3rd Floor Main Library

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Call Number: A1234.567
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