Overcoming the Specific Toxicity of Large Plasmids Electrotransfer in Primary Cells In Vitro
Gene electrotransfer is a safe and efficient nonviral technique for the transfer of nucleic acids of all sizes. Using a small reporter plasmid (3.5 kbp), electrotransfer of more than 90% of the cells, with ≃70% viability, can be routinely achieved even in primary cells like mesenchymal stem cells. H...
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Format: | Book |
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Elsevier,
2016-01-01T00:00:00Z.
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A1234.567 |
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