Overcoming the Specific Toxicity of Large Plasmids Electrotransfer in Primary Cells In Vitro

Gene electrotransfer is a safe and efficient nonviral technique for the transfer of nucleic acids of all sizes. Using a small reporter plasmid (3.5 kbp), electrotransfer of more than 90% of the cells, with ≃70% viability, can be routinely achieved even in primary cells like mesenchymal stem cells. H...

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Main Authors: Léa L Lesueur (Author), Lluis M Mir (Author), Franck M André (Author)
Format: Book
Published: Elsevier, 2016-01-01T00:00:00Z.
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3rd Floor Main Library

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Call Number: A1234.567
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