Efficient shRNA-based knockdown of multiple target genes for cell therapy using a chimeric miRNA cluster platform

• Main Authors: Matteo Rossi (Author), Mikhail Steklov (Author), Fanny Huberty (Author), Thuy Nguyen (Author), Jérôme Marijsse (Author), Céline Jacques-Hespel (Author), Paul Najm (Author), Caroline Lonez (Author), Eytan Breman (Author)
• Format: Book
• Published: Elsevier, 2023-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Genome engineering technologies are powerful tools in cell-based immunotherapy to optimize or fine-tune cell functionalities. However, their use for multiple gene edits poses relevant biological and technical challenges. Short hairpin RNA (shRNA)-based cell engineering bypasses these criticalities a...