Efficient shRNA-based knockdown of multiple target genes for cell therapy using a chimeric miRNA cluster platform

• Glavni autori: Matteo Rossi (Autor), Mikhail Steklov (Autor), Fanny Huberty (Autor), Thuy Nguyen (Autor), Jérôme Marijsse (Autor), Céline Jacques-Hespel (Autor), Paul Najm (Autor), Caroline Lonez (Autor), Eytan Breman (Autor)
• Format: Knjiga
• Izdano: Elsevier, 2023-12-01T00:00:00Z.
• Teme: article
• Online pristup: Connect to this object online.

Genome engineering technologies are powerful tools in cell-based immunotherapy to optimize or fine-tune cell functionalities. However, their use for multiple gene edits poses relevant biological and technical challenges. Short hairpin RNA (shRNA)-based cell engineering bypasses these criticalities a...