Sequential Accumulation of 'Driver' Pathway Mutations Induces the Upregulation of Hydrogen-Sulfide-Producing Enzymes in Human Colonic Epithelial Cell Organoids

• Main Authors: Kelly Ascenção (Author), Nahzli Dilek (Author), Karim Zuhra (Author), Katalin Módis (Author), Toshiro Sato (Author), Csaba Szabo (Author)
• Format: Knjiga
• Izdano: MDPI AG, 2022-09-01T00:00:00Z.
• Teme: article
• Online dostop: Connect to this object online.

Recently, a CRISPR-Cas9 genome-editing system was developed with introduced sequential 'driver' mutations in the WNT, MAPK, TGF-β, TP53 and PI3K pathways into organoids derived from normal human intestinal epithelial cells. Prior studies have demonstrated that isogenic organoids harboring...