Sequential Accumulation of 'Driver' Pathway Mutations Induces the Upregulation of Hydrogen-Sulfide-Producing Enzymes in Human Colonic Epithelial Cell Organoids

• Glavni autori: Kelly Ascenção (Autor), Nahzli Dilek (Autor), Karim Zuhra (Autor), Katalin Módis (Autor), Toshiro Sato (Autor), Csaba Szabo (Autor)
• Format: Knjiga
• Izdano: MDPI AG, 2022-09-01T00:00:00Z.
• Teme: article
• Online pristup: Connect to this object online.

Recently, a CRISPR-Cas9 genome-editing system was developed with introduced sequential 'driver' mutations in the WNT, MAPK, TGF-β, TP53 and PI3K pathways into organoids derived from normal human intestinal epithelial cells. Prior studies have demonstrated that isogenic organoids harboring...