Combining an optimized mRNA template with a double purification process allows strong expression of in vitro transcribed mRNA

• Main Authors: Sergio Linares-Fernández (Author), Julien Moreno (Author), Elise Lambert (Author), Perrine Mercier-Gouy (Author), Laetitia Vachez (Author), Bernard Verrier (Author), Jean-Yves Exposito (Author)
• Format: Book
• Published: Elsevier, 2021-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

mRNA is a blooming technology for vaccination and has gained global attention during the SARS-CoV-2 pandemic. However, the recent clinical trials have highlighted increased reactogenicity when using high mRNA doses. Intending to increase the potency of mRNA therapeutics and to decrease the therapeut...