Molecular Modification of Kex2 P1' Site Enhances Expression and Druggability of Fungal Defensin

• Main Authors: Yanjie Jin (Author), Na Yang (Author), Da Teng (Author), Ya Hao (Author), Ruoyu Mao (Author), Jianhua Wang (Author)
• Format: Book
• Published: MDPI AG, 2023-04-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

<i>Pichia pastoris</i> is the widely used expression system for producing recombinant secretory proteins. It is known that Kex2 protease plays a vital role in the process of protein secretion, in which the P1' site affects its cleavage efficiency. To enhance the expression level of...