Nicotine- and Tar-Free Cigarette Smoke Induces Cell Damage Through Reactive Oxygen Species Newly Generated by PKC-Dependent Activation of NADPH Oxidase

• Main Authors: Hiroshi Asano (Author), Takahiro Horinouchi (Author), Yosuke Mai (Author), Osamu Sawada (Author), Shunsuke Fujii (Author), Tadashi Nishiya (Author), Masabumi Minami (Author), Takahiro Katayama (Author), Toshihiko Iwanaga (Author), Koji Terada (Author), Soichi Miwa (Author)
• Format: Book
• Published: Elsevier, 2012-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

We examined cytotoxic effects of nicotine/tar-free cigarette smoke extract (CSE) on C6 glioma cells. The CSE induced plasma membrane damage (determined by lactate dehydrogenase leakage and propidium iodide uptake) and cell apoptosis {determined by MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethox...