Treatment of CHO cells with Taxol and reversine improves micronucleation and microcell-mediated chromosome transfer efficiency

• Main Authors: Narumi Uno (Author), Hiroyuki Satofuka (Author), Hitomaru Miyamoto (Author), Kazuhisa Honma (Author), Teruhiko Suzuki (Author), Kyotaro Yamazaki (Author), Ryota Ito (Author), Takashi Moriwaki (Author), Shusei Hamamichi (Author), Kazuma Tomizuka (Author), Mitsuo Oshimura (Author), Yasuhiro Kazuki (Author)
• Format: Book
• Published: Elsevier, 2023-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Microcell-mediated chromosome transfer is an attractive technique for transferring chromosomes from donor cells to recipient cells and has enabled the generation of cell lines and humanized animal models that contain megabase-sized gene(s). However, improvements in chromosomal transfer efficiency ar...